Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.
Copyright policy )Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.
The detailed genomic organization of a gene-dense region at human chromosome 12p13, spanning 223 kb of contiguous sequence, was determined. This region is composed of 20 genes and several other expressed sequences. Experimental tools including RT-PCR and cDNA sequencing, combined with gene prediction programs, were utilized in the analysis of the sequence. Various computer software programs were employed for sequence similarity searches and functional predictions. The high number of genes with diverse functions and complex transcriptional patterns make this region ideal for addressing challenges of gene discovery and genomic characterization amenable to large-scale sequence analysis.
- Baylor College of Medicine United States
Genomic Library, Chromosomes, Human, Pair 12, DNA, Complementary, Sequence Homology, Amino Acid, Molecular Sequence Data, Chromosome Mapping, Computational Biology, Gene Expression, Nerve Tissue Proteins, RNA-Directed DNA Polymerase, Exons, Sequence Analysis, DNA, Polymerase Chain Reaction, Introns, Alternative Splicing, Multigene Family, Humans, Amino Acid Sequence, Glycolysis, Signal Transduction
Genomic Library, Chromosomes, Human, Pair 12, DNA, Complementary, Sequence Homology, Amino Acid, Molecular Sequence Data, Chromosome Mapping, Computational Biology, Gene Expression, Nerve Tissue Proteins, RNA-Directed DNA Polymerase, Exons, Sequence Analysis, DNA, Polymerase Chain Reaction, Introns, Alternative Splicing, Multigene Family, Humans, Amino Acid Sequence, Glycolysis, Signal Transduction
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