Hippocampal Neurons Express a Calcineurin-Activated Adenylyl Cyclase
Copyright policy )Hippocampal Neurons Express a Calcineurin-Activated Adenylyl Cyclase
Ca2+-stimulated adenylyl cyclases are important for several forms of neuroplasticity because they couple activity-dependent Ca2+increases to cAMP in neurons. For example, the calmodulin-stimulated adenylyl cyclases, AC1 and AC8, are required for hippocampus-dependent memory and long-lasting long-term potentiation. To identify other mechanisms for Ca2+stimulation of adenylyl cyclases, cultured hippocampal neurons from transgenic mice lacking both AC1 and AC8 [double knock-out (DKO) mice] were analyzed for Ca2+stimulation of intracellular cAMP. Surprisingly, neurons from DKO mice showed significant Ca2+-stimulated cAMP accumulation that was blocked by inhibitors of calcineurin [PP2B (protein phosphatase 2B)], a Ca2+-activated protein phosphatase. Analysis of cultured neurons from calcineurin-/-mice confirmed that hippocampal neurons exhibit a calcineurin-dependent cAMP increase, which may contribute to some forms of neuroplasticity.
- Massachusetts Institute of Technology United States
- University of Mary United States
- University of Washington United States
Mice, Knockout, Neurons, Dose-Response Relationship, Drug, Calcineurin, Hippocampus, Potassium Chloride, Enzyme Activation, Mice, Inbred C57BL, Mice, Animals, Newborn, Cyclic AMP, Animals, Calcium, Drug Interactions, Enzyme Inhibitors, Cells, Cultured, Adenylyl Cyclases
Mice, Knockout, Neurons, Dose-Response Relationship, Drug, Calcineurin, Hippocampus, Potassium Chloride, Enzyme Activation, Mice, Inbred C57BL, Mice, Animals, Newborn, Cyclic AMP, Animals, Calcium, Drug Interactions, Enzyme Inhibitors, Cells, Cultured, Adenylyl Cyclases
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