Molecular Genetic Tests for FLT3, NPM1, and CEBPA in Acute Myeloid Leukemia
Molecular Genetic Tests for FLT3, NPM1, and CEBPA in Acute Myeloid Leukemia
Patients with acute myeloid leukemia (AML) and a normal karyotype constitute the single largest cytogenetic group of AML. It is important to identify prognostic markers that predict patients' outcome more precisely. The presence of mutations in FLT3 (FMS-like tyrosine kinase 3), NPM1 (Nucleophosmin), and CEBPA (CCAAT/enhancer-binding protein alpha) genes hold prognostic significance in patients with AML and normal cytogenetics. Therefore, mutation identification may help to optimize therapeutic approaches in this group of patients. Polymerase chain reaction (PCR)-based fragment length analysis for mutations in FLT3 and NPM1 has been shown to be a fast and sensitive method, while nucleotide sequencing represents a gold standard for CEBPA heterogeneous mutational screening. We describe both fragment length assay and sequencing methods for mutational analysis of these three genes.
- Indiana University School of Medicine United States
- Indiana University United States
Gene Expression Regulation, Leukemic, DNA Mutational Analysis, Karyotype, Nuclear Proteins, Sequence Analysis, DNA, Prognosis, Leukemia, Myeloid, Acute, fms-Like Tyrosine Kinase 3, Mutation, CCAAT-Enhancer-Binding Proteins, Humans, Female, Genetic Testing, Nucleophosmin
Gene Expression Regulation, Leukemic, DNA Mutational Analysis, Karyotype, Nuclear Proteins, Sequence Analysis, DNA, Prognosis, Leukemia, Myeloid, Acute, fms-Like Tyrosine Kinase 3, Mutation, CCAAT-Enhancer-Binding Proteins, Humans, Female, Genetic Testing, Nucleophosmin
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!