Maturation of postnatally generated olfactory bulb granule cells depends on functional γ-protocadherin expression
Copyright policy )Maturation of postnatally generated olfactory bulb granule cells depends on functional γ-protocadherin expression
γ-protocadherins (γ-pcdhs) are transmembrane receptor proteins ubiquitously expressed in the postnatal and adult mouse brain. γ-pcdhs are required for normal neuronal development as shown for spinal cord interneurons, retinal ganglion cells and cortical neurons. To test the role of γ-pcdhs during development of subventricular zone progenitor cells and their subsequent differentiation into olfactory granule cells we generated a conditional γ-pcdh(lox/lox) allele (γ-pcdh(lox/lox)) allowing for functional γ-pcdh inactivation upon lentivirus-mediated Cre-recombinase expression selectively in subventricular zone progenitor cells. While γ-pcdh loss did not alter the proliferation of subventricular zone progenitors, γ-pcdh ko progenitors that reached the main olfactory bulb showed a significant reduction in dendritic arborization and failed to develop dendritic spines. Our results suggest that olfactory bulb granule cell maturation necessitates functional γ-pcdh expression.
- Max Planck Society Germany
- Max Planck Institute for Medical Research Germany
Male, Mice, Knockout, Integrases, Genetic Vectors, Green Fluorescent Proteins, Lentivirus, Cadherin Related Proteins, Gene Expression Regulation, Developmental, Dendrites, Cadherins, Article, Mice, Inbred C57BL, Mice, Cell Movement, Genes, Reporter, Animals, Cell Lineage, Female, Cell Shape, Cell Division, Injections, Intraventricular
Male, Mice, Knockout, Integrases, Genetic Vectors, Green Fluorescent Proteins, Lentivirus, Cadherin Related Proteins, Gene Expression Regulation, Developmental, Dendrites, Cadherins, Article, Mice, Inbred C57BL, Mice, Cell Movement, Genes, Reporter, Animals, Cell Lineage, Female, Cell Shape, Cell Division, Injections, Intraventricular
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