Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein
Copyright policy )Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein
The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2.
- New York University Langone Medical Center United States
- New York University United States
Models, Molecular, Binding Sites, Base Sequence, Macromolecular Substances, Protein Conformation, Molecular Sequence Data, Oncogene Proteins, Viral, Crystallography, X-Ray, Protein Structure, Secondary, DNA-Binding Proteins, Viral Proteins, DNA, Viral, Animals, Humans, Nucleic Acid Conformation, Cattle, Amino Acid Sequence, Dimerization, Bovine papillomavirus 1, Protein Binding
Models, Molecular, Binding Sites, Base Sequence, Macromolecular Substances, Protein Conformation, Molecular Sequence Data, Oncogene Proteins, Viral, Crystallography, X-Ray, Protein Structure, Secondary, DNA-Binding Proteins, Viral Proteins, DNA, Viral, Animals, Humans, Nucleic Acid Conformation, Cattle, Amino Acid Sequence, Dimerization, Bovine papillomavirus 1, Protein Binding
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