Role of cytosolic and calcium independent phospholipases A2 in insulin secretion impairment of INS‐1E cells infected by S. aureus
Copyright policy )Role of cytosolic and calcium independent phospholipases A2 in insulin secretion impairment of INS‐1E cells infected by S. aureus
Cytosolic PLA2 (cPLA2) and Ca2+‐independent PLA2 (iPLA2) play a significant role in insulin β‐cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which Staphylococcus aureus infection of INS‐1 cells alters glucose‐induced insulin secretion has been examined. After acute infection, insulin secretion and PLA2 activities significantly increased. Moreover, increased expressions of phospho‐cPLA2, phospho‐PKCα and phospho‐ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA2 and COX‐2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific siRNAs against iPLA2 isoform and specific COX‐2 inhibitor.
- University of Catania Italy
Methicillin-Resistant Staphylococcus aureus, Protein Kinase C-alpha, Cyclooxygenase 2 Inhibitors, MAP Kinase Signaling System, Group IV Phospholipases A2, Rats, Group VI Phospholipases A2, Kinetics, Phospholipases A2; INS-1E cell ; Insulin ; siRNA; Staphylococcus aureus, Diabetes Mellitus, Type 1, Pancreatitis, Cyclooxygenase 2, Cell Line, Tumor, Insulin-Secreting Cells, Host-Pathogen Interactions, Insulin Secretion, Animals, Insulin, RNA Interference, Phosphorylation, Protein Processing, Post-Translational
Methicillin-Resistant Staphylococcus aureus, Protein Kinase C-alpha, Cyclooxygenase 2 Inhibitors, MAP Kinase Signaling System, Group IV Phospholipases A2, Rats, Group VI Phospholipases A2, Kinetics, Phospholipases A2; INS-1E cell ; Insulin ; siRNA; Staphylococcus aureus, Diabetes Mellitus, Type 1, Pancreatitis, Cyclooxygenase 2, Cell Line, Tumor, Insulin-Secreting Cells, Host-Pathogen Interactions, Insulin Secretion, Animals, Insulin, RNA Interference, Phosphorylation, Protein Processing, Post-Translational
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