Large Scale Localization of Protein Phosphorylation by Use of Electron Capture Dissociation Mass Spectrometry
Copyright policy )Large Scale Localization of Protein Phosphorylation by Use of Electron Capture Dissociation Mass Spectrometry
We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment.
- University of Birmingham United Kingdom
- University of Birmingham, School of Biosciences United Kingdom
- University Of Birmingham
- University of Birmingham
Phosphopeptides, Research, Molecular Sequence Data, Proteins, Reproducibility of Results, Q Science (General), Mass Spectrometry, Mice, NIH 3T3 Cells, Animals, Amino Acid Sequence, Phosphorylation
Phosphopeptides, Research, Molecular Sequence Data, Proteins, Reproducibility of Results, Q Science (General), Mass Spectrometry, Mice, NIH 3T3 Cells, Animals, Amino Acid Sequence, Phosphorylation
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).79 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!