Biochemical and genetic characterization of esterase-27 (ES-27), the major plasma cholinesterase of the house mouse (Mus musculus)
pmid: 2040456
Biochemical and genetic characterization of esterase-27 (ES-27), the major plasma cholinesterase of the house mouse (Mus musculus)
SummaryEsterase-27A (ES-27A) was characterized in strain A/WySnA by a cascade of seven bands seen after disc electrophoresis of serum and subsequent staining for esterase. ES-27A catalyses the hydrolysis of thiocholine butyrate and is strongly inhibited by 100 μm tetraisopropyl pyrophosphamide (isoOMPA). Hence, the enzyme was concluded to be a cholinesterase EC 3.1.1.8. A heat-labile form termed ES-27B was represented by strain AKR/Han. From a three-point cross (AKR/Han, A/Wy) and a five-point cross (AKR/Han, SEG/1), the gene order on chromosome 3 was concluded to be centromere–Car-2–Es-26–Es-27–Amy-1–Adh-1.
- Pathologisches Institut Germany
- University of Freiburg Germany
Male, Mice, Animals, Cholinesterases, Chromosome Mapping, Female, Mice, Inbred Strains
Male, Mice, Animals, Cholinesterases, Chromosome Mapping, Female, Mice, Inbred Strains
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