Increased expression and altered location of annexin IV in renal clear cell carcinoma: a possible role in tumour dissemination
Copyright policy )Increased expression and altered location of annexin IV in renal clear cell carcinoma: a possible role in tumour dissemination
The proteome of renal cell carcinoma and non-neoplastic kidney tissue was analysed from 12 patients by two-dimensional polyacrylamide gel electrophoresis to search for differentially expressed proteins in the tumour. Annexin IV was identified to be up-regulated in tumour cells. These patients and further 11 were characterized by RT-PCR. We found an increased amount of annexin IV mRNA. Immunohistochemical analysis revealed an altered localization of annexin IV in tumour cells. Additionally we demonstrate that over-expressed annexin IV promotes cell migration in a carcinoma model system. From these results above it seems possible that annexin IV plays an important role in the morphological diversification and dissemination of the clear cell renal cell carcinoma.
- University of Greifswald Germany
Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Immunohistochemistry, Kidney Neoplasms, Up-Regulation, Luminescent Proteins, Cell Movement, Humans, Electrophoresis, Gel, Two-Dimensional, Neoplasm Invasiveness, RNA, Messenger, Annexin A4, Carcinoma, Renal Cell, Adenocarcinoma, Clear Cell
Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Immunohistochemistry, Kidney Neoplasms, Up-Regulation, Luminescent Proteins, Cell Movement, Humans, Electrophoresis, Gel, Two-Dimensional, Neoplasm Invasiveness, RNA, Messenger, Annexin A4, Carcinoma, Renal Cell, Adenocarcinoma, Clear Cell
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