Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes
Copyright policy )Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes
Abstract Multidrug resistant (MDR) Acinetobacter baumannii poses a growing threat to global health. Research on Acinetobacter pathogenesis has primarily focused on pneumonia and bloodstream infections, even though one in five A. baumannii strains are isolated from urinary sites. In this study, we highlight the role of A. baumannii as a uropathogen. We develop the first A. baumannii catheter-associated urinary tract infection (CAUTI) murine model using UPAB1, a recent MDR urinary isolate. UPAB1 carries the plasmid pAB5, a member of the family of large conjugative plasmids that represses the type VI secretion system (T6SS) in multiple Acinetobacter strains. pAB5 confers niche specificity, as its carriage improves UPAB1 survival in a CAUTI model and decreases virulence in a pneumonia model. Comparative proteomic and transcriptomic analyses show that pAB5 regulates the expression of multiple chromosomally-encoded virulence factors besides T6SS. Our results demonstrate that plasmids can impact bacterial infections by controlling the expression of chromosomal genes.
- Seattle Institute for Biomedical and Clinical Research United States
- University of Mary United States
- Washington University in St. Louis School of Medicine United States
- Department of Pathology and Immunology Washington University School of Medicine United States
- Universidid De Buenos Aires Argentina
Proteomics, Acinetobacter baumannii, 570, Science, 610, FOS: Basic medicine, Urinary Catheters, Plasmid, Microbiology, Gene, Article, Global Challenge of Antibiotic Resistance in Bacteria, Mice, Endocrinology, Bacterial Proteins, Antibiotics, Drug Resistance, Multiple, Bacterial, Biochemistry, Genetics and Molecular Biology, Pneumonia, Bacterial, Genetics, Animals, Humans, Urinary Tract, Pathogenesis and Virulence of Escherichia coli, Biology, Retrospective Studies, Dynamics and Pathogenesis of Cholera Bacteria, Virulence, Acinetobacter, Bacteria, Gene Expression Profiling, Q, Life Sciences, Gene Expression Regulation, Bacterial, Type VI Secretion Systems, Disease Models, Animal, Catheter-Related Infections, FOS: Biological sciences, Urinary Tract Infections, Pseudomonas aeruginosa, Molecular Medicine, Gene expression, Transcriptome, Acinetobacter Infections, Plasmids
Proteomics, Acinetobacter baumannii, 570, Science, 610, FOS: Basic medicine, Urinary Catheters, Plasmid, Microbiology, Gene, Article, Global Challenge of Antibiotic Resistance in Bacteria, Mice, Endocrinology, Bacterial Proteins, Antibiotics, Drug Resistance, Multiple, Bacterial, Biochemistry, Genetics and Molecular Biology, Pneumonia, Bacterial, Genetics, Animals, Humans, Urinary Tract, Pathogenesis and Virulence of Escherichia coli, Biology, Retrospective Studies, Dynamics and Pathogenesis of Cholera Bacteria, Virulence, Acinetobacter, Bacteria, Gene Expression Profiling, Q, Life Sciences, Gene Expression Regulation, Bacterial, Type VI Secretion Systems, Disease Models, Animal, Catheter-Related Infections, FOS: Biological sciences, Urinary Tract Infections, Pseudomonas aeruginosa, Molecular Medicine, Gene expression, Transcriptome, Acinetobacter Infections, Plasmids
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