Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli
Copyright policy )Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli
ABSTRACT Putative hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound. High expression of hopA and hopB (encoding squalene/phytoene synthases) together with hopD (encoding farnesyl diphosphate synthase) yielded 4.1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi , E. coli genes encoding 1-deoxy- d -xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase.
- Sun Moon University Korea (Republic of)
Squalene, Alkyl and Aryl Transferases, Escherichia coli Proteins, Molecular Sequence Data, Geranyltranstransferase, Gene Expression Regulation, Bacterial, Carbon-Carbon Double Bond Isomerases, Streptomyces, Farnesyl-Diphosphate Farnesyltransferase, Hemiterpenes, Bacterial Proteins, Transferases, Geranylgeranyl-Diphosphate Geranylgeranyltransferase, Escherichia coli, Biotechnology
Squalene, Alkyl and Aryl Transferases, Escherichia coli Proteins, Molecular Sequence Data, Geranyltranstransferase, Gene Expression Regulation, Bacterial, Carbon-Carbon Double Bond Isomerases, Streptomyces, Farnesyl-Diphosphate Farnesyltransferase, Hemiterpenes, Bacterial Proteins, Transferases, Geranylgeranyl-Diphosphate Geranylgeranyltransferase, Escherichia coli, Biotechnology
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