MDC1 Regulates DNA-PK Autophosphorylation in Response to DNA Damage
Copyright policy )MDC1 Regulates DNA-PK Autophosphorylation in Response to DNA Damage
DNA damage initiates signaling events through kinase cascades that result in cell cycle checkpoint control and DNA repair. However, it is not yet clear how the signaling pathways relay to DNA damage repair. Using the repeat region of checkpoint protein MDC1 (mediator of DNA damage checkpoint protein 1), we identified DNA-PKcs/Ku as MDC1-associated proteins. Here, we show that MDC1 directly interacts with the Ku/DNA-PKcs complex. Down-regulation of MDC1 resulted in defective phospho-DNA-PKcs foci formation and DNA-PKcs autophosphorylation, suggesting that MDC1 regulates autophosphorylation of DNA-PKcs following DNA damage. Furthermore, DNA-PK-dependent DNA damage repair is defective in cells depleted of MDC1. Taken together, these results suggest that the MDC1 repeat region is involved in protein-protein interaction with DNA-PKcs/Ku, and MDC1 regulates DNA damage repair by influencing DNA-PK autophosphorylation. Therefore, MDC1 acts not only as a mediator of DNA damage checkpoint but also as a mediator of DNA damage repair.
- Mayo Clinic United States
- The University of Texas Southwestern Medical Center United States
- Lawrence Berkeley National Laboratory United States
DNA Repair, Cell Cycle, Down-Regulation, Nuclear Proteins, Cell Cycle Proteins, DNA, DNA-Activated Protein Kinase, Protein Serine-Threonine Kinases, Cell Line, Protein Structure, Tertiary, DNA-Binding Proteins, Microscopy, Fluorescence, Humans, Phosphorylation, RNA, Small Interfering, Adaptor Proteins, Signal Transducing, DNA Damage, Glutathione Transferase, HeLa Cells, Protein Binding
DNA Repair, Cell Cycle, Down-Regulation, Nuclear Proteins, Cell Cycle Proteins, DNA, DNA-Activated Protein Kinase, Protein Serine-Threonine Kinases, Cell Line, Protein Structure, Tertiary, DNA-Binding Proteins, Microscopy, Fluorescence, Humans, Phosphorylation, RNA, Small Interfering, Adaptor Proteins, Signal Transducing, DNA Damage, Glutathione Transferase, HeLa Cells, Protein Binding
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