Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways
Copyright policy )Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways
It is well known that trauma is frequently accompanied by spontaneous functional recovery after spinal cord injury (SCI), but the underlying mechanisms remain elusive. In this study, BBB scores showed a gradual return of locomotor functions after SCT. Proteomics analysis revealed 16 differential protein spots in the gastrocnemius muscle between SCT and normal rats. Of these differential proteins, eukaryotic translation initiation factor 5A1 (elf-5A1), a highly conserved molecule throughout eukaryotes, exhibited marked upregulation in the gastrocnemius muscle after SCT. To study the role of eIF-5A1 in the restoration of hindlimb locomotor functions following SCT, we used siRNA to downregulate the mRNA level of eIF-5A1. Compared with untreated SCT control rats, those subjected to eIF-5A1 knockdown exhibited impaired functional recovery. Moreover, gene expression microarrays and bioinformatic analysis showed high correlation between three main signal pathways (ErbB, MAPK and neurotrophin signal pathways) and eIF-5A1. These signal pathways regulate cell proliferation, differentiation and neurocyte growth. Consequently, eIF-5A1 played a pivotal role via these signal pathways in hindlimb locomotor functional recovery after SCT, which could pave the way for the development of a new strategy for the treatment of spinal cord injury in clinical trials.
- Yunnan Agricultural University China (People's Republic of)
- University of South Australia Australia
- Kunming Medical University China (People's Republic of)
- Sichuan University China (People's Republic of)
Proteomics, MAP Kinase Signaling System, Movement, Rats, Sprague-Dawley, proteomics, Peptide Initiation Factors, Animals, Nerve Growth Factors, skeletal muscle, RNA, Small Interfering, Muscle, Skeletal, Cell Proliferation, Gene Expression Profiling, Muscles, Computational Biology, RNA-Binding Proteins, Cell Differentiation, Oncogene Proteins v-erbB, Hindlimb, Rats, spinal cord transection, RNAi, gene expression microarray, Female, RNA Interference
Proteomics, MAP Kinase Signaling System, Movement, Rats, Sprague-Dawley, proteomics, Peptide Initiation Factors, Animals, Nerve Growth Factors, skeletal muscle, RNA, Small Interfering, Muscle, Skeletal, Cell Proliferation, Gene Expression Profiling, Muscles, Computational Biology, RNA-Binding Proteins, Cell Differentiation, Oncogene Proteins v-erbB, Hindlimb, Rats, spinal cord transection, RNAi, gene expression microarray, Female, RNA Interference
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!