SIR‐dependent repression of non‐telomeric genes in Saccharomyces cerevisiae?
Copyright policy )SIR‐dependent repression of non‐telomeric genes in Saccharomyces cerevisiae?
AbstractThe proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating‐type loci HML and HMR and of reporter genes inserted at telomeres. Previous microarray analyses suggested that additional non‐telomeric genes exist which are repressed by the Sir proteins. In this study, we tested the expression of 12 such genes by Northern analysis and RT–PCR. However, we were unable to verify their SIR‐dependent regulation, which suggests that SIR‐mediated repression may be restricted to the known repressed regions. Copyright © 2003 John Wiley & Sons, Ltd.
- Max Planck Society Germany
- Max Planck Institute for Molecular Genetics Germany
Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Genes, Fungal, Chemie, RNA, Fungal, Saccharomyces cerevisiae, Telomere, Blotting, Northern, Genes, Mating Type, Fungal, Gene Expression Regulation, Fungal, Biologie, Silent Information Regulator Proteins, Saccharomyces cerevisiae
Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Genes, Fungal, Chemie, RNA, Fungal, Saccharomyces cerevisiae, Telomere, Blotting, Northern, Genes, Mating Type, Fungal, Gene Expression Regulation, Fungal, Biologie, Silent Information Regulator Proteins, Saccharomyces cerevisiae
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