Complex regulation of Calbindin-D9k in the mouse placenta and extra-embryonic membrane during mid- and late pregnancy
Copyright policy )Complex regulation of Calbindin-D9k in the mouse placenta and extra-embryonic membrane during mid- and late pregnancy
Calbindin-D(9k) (CaBP-9k) is a cytosolic calcium-binding protein mainly expressed in the duodenum, uterus and placenta, however, the role of CaBP-9k in the regulation of fetal growth remains to be elucidated. The present study was performed to investigate the expression pattern and regulation of CaBP-9k by antagonists of steroid hormones related with steroid hormone receptors during mid- and late pregnancy in mouse placenta and extra-embryonic membrane. The expression level of CaBP-9k increased in the placenta, while it decreased in the extra-embryonic membrane during pregnancy. The mRNA expression levels of estrogen receptor alpha (ERalpha) and progesterone receptor (PR) appeared to increase in both placenta and extra-embryonic membrane during pregnancy, suggesting that the ER and PR mRNA and protein expressions of placental CaBP-9k are positively correlated, but expressions of extra-embryonic membrane CaBP-9k are reversely correlated with ERalpha and PR mRNA levels. In addition, the present study indicates that the expressions of CaBP-9k mRNA and protein are differentially up- or down-regulated by antagonists of estrogen (E2) and progesterone (P4) in mouse placenta and extra-embryonic membranes, which suggests that E2 and P4 may be dominant factors in the regulation of the CaBP-9k. In particular, RU486, an antagonist of P4, down-regulated the mRNA and protein levels of placental CaBP-9k, whereas it up-regulated the protein level of extra-embryonic membrane CaBP-9k. In conclusion, we demonstrated that the CaBP-9k is distinctly regulated in the mouse placenta and extra-embryonic membrane, probably via sex steroid hormones (E2 and P4) and their receptors through a complex pathway. Extended studies are needed to verify relevant factors to regulate CaBP-9k gene and to provide further insight into roles of CaBP-9k gene in these tissues for the control of reproductive functions.
- University of British Columbia Canada
- Seoul National University Korea (Republic of)
- Chungbuk National University Korea (Republic of)
- British Columbia Children's Hospital Canada
Calbindins, Mice, Inbred ICR, Time Factors, Estradiol, Placenta, Estrogen Receptor alpha, Extraembryonic Membranes, Gene Expression Regulation, Developmental, Mice, S100 Calcium Binding Protein G, Receptors, Estrogen, Pregnancy, Animals, Female, RNA, Messenger, Receptors, Progesterone, Progesterone
Calbindins, Mice, Inbred ICR, Time Factors, Estradiol, Placenta, Estrogen Receptor alpha, Extraembryonic Membranes, Gene Expression Regulation, Developmental, Mice, S100 Calcium Binding Protein G, Receptors, Estrogen, Pregnancy, Animals, Female, RNA, Messenger, Receptors, Progesterone, Progesterone
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