Expression and localization of VEGF receptors in human fetal skeletal tissues.
Copyright policy )Expression and localization of VEGF receptors in human fetal skeletal tissues.
During development the vertebrate skeleton is the product of deriving cells from distinct embryonic lineages. The craniofacial skeleton is formed by migrating cranial neural crest cells, whereas the axial and limb skeletons are derived from mesodermal cells. The Vascular Endothelial Growth Factors (VEGFs) / receptors (VEGFRs) system plays an important role in angiogenesis, as well as osteogenesis, during bone development, growth, and remodeling, attracting endothelial cells and osteoclasts and stimulating osteoblast differentiation. Recent evidence has shown that during development VEGFR-3 is also expressed in neural and glial precursors of forebrain and cerebellum, as well as in the eye. In this study, we found that VEGFR-1, VEGFR-2 and VEGFR-3 are expressed in human bone both in fetal and adult life. The gene expression levels were significantly higher in fetal samples especially in mandibles. In addition, higher levels of VEGFR-3 in orofacial district were confirmed by western blotting analysis. We also observed that in fetal mandibular samples VEGFRs colocalized in several osteoblasts, osteoclasts and osteoprogenitor cells. Furthermore, some cells coexpressed VEGFR-3 and ET-1, a marker of neural crest cells. The results demonstrated different expression of VEGFRs in human mandibular and femoral bones which could be correlated to their different structure, function and development during organogenesis. VEGFR-3 might represent a specific signal for ectomesenchymal lineage differentiation during early human development.
- New York University Italy
- University of Molise Italy
- University of Florence Italy
Adult, Mandible, Real-Time Polymerase Chain Reaction, Bone and Bones, Fetus, Skeletogenesis, Humans, Femur, RNA, Messenger, Bone Development, Microscopy, Confocal, Vascular Endothelial Growth Factor Receptor-1, Reverse Transcriptase Polymerase Chain Reaction, VEGFRs, VEGFR3, skeletogenesis, development, neural crest, Gene Expression Regulation, Developmental, Middle Aged, Vascular Endothelial Growth Factor Receptor-3, Immunohistochemistry, Vascular Endothelial Growth Factor Receptor-2, CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología, Receptors, Vascular Endothelial Growth Factor, :5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología [CDU], VEGFRs
Adult, Mandible, Real-Time Polymerase Chain Reaction, Bone and Bones, Fetus, Skeletogenesis, Humans, Femur, RNA, Messenger, Bone Development, Microscopy, Confocal, Vascular Endothelial Growth Factor Receptor-1, Reverse Transcriptase Polymerase Chain Reaction, VEGFRs, VEGFR3, skeletogenesis, development, neural crest, Gene Expression Regulation, Developmental, Middle Aged, Vascular Endothelial Growth Factor Receptor-3, Immunohistochemistry, Vascular Endothelial Growth Factor Receptor-2, CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología, Receptors, Vascular Endothelial Growth Factor, :5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología [CDU], VEGFRs
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