Co‐expressed recombinant human Translin‐Trax complex binds DNA
Copyright policy )Co‐expressed recombinant human Translin‐Trax complex binds DNA
Trax, expressed alone aggregates into insoluble complexes, whereas upon co‐expression with Translin becomes readily soluble and forms a stable heteromeric complex (∼430 kDa) containing both proteins at nearly equimolar ratio. Based on the subunit molecular weights, estimated by MALDI‐TOF‐MS, the purified complex appears to comprise of either an octameric Translin plus a hexameric Trax (calculated MW 420 kDa) or a heptamer each of Trax and Translin (calculated MW 425 kDa) or a hexameric Translin plus an octameric Trax (calculated MW 431 kDa). The complex binds single‐stranded/double‐stranded DNA. ssDNA gel‐shifted complex shows both proteins at nearly equimolar ratio, suggesting that Translin “chaperones” Trax and forms heteromeric complex that is DNA binding competent.
DNA-binding activity, Gene Expression, DNA, Co-expression, Recombinant Proteins, DNA-Binding Proteins, Trax-Translin complex, Oligomers, Multiprotein Complexes, Escherichia coli, Humans, Purification, Protein Binding
DNA-binding activity, Gene Expression, DNA, Co-expression, Recombinant Proteins, DNA-Binding Proteins, Trax-Translin complex, Oligomers, Multiprotein Complexes, Escherichia coli, Humans, Purification, Protein Binding
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