Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β
Copyright policy )Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β
The K + channel KCa3.1 is required for Ca 2+ influx and the subsequent activation of CD4 T cells. The class II phosphatidylinositol 3 kinase C2β (PI3KC2β) is activated by the T-cell receptor (TCR) and is critical for KCa3.1 channel activation. Tripartite motif containing protein 27 (TRIM27) is a member of a large family of proteins that function as Really Interesting New Gene (RING) E3 ubiquitin ligases. We now show that TRIM27 functions as an E3 ligase and mediates lysine 48 polyubiquitination of PI3KC2β, leading to a decrease in PI3K enzyme activity. By inhibiting PI3KC2β, TRIM27 also functions to negatively regulate CD4 T cells by inhibiting KCa3.1 channel activity and TCR-stimulated Ca 2+ influx and cytokine production in Jurkat, primary human CD4 T cells, and Th0, Th1, and Th2 CD4 T cells generated from TRIM27 −/− mice. These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells.
- New York University United States
- Boehringer Ingelheim Fonds Germany
- Yeshiva University United States
- New York University Langone Medical Center United States
- Boehringer Ingelheim (United States) United States
CD4-Positive T-Lymphocytes, Receptors, Antigen, T-Cell, Nuclear Proteins, Th1 Cells, Intermediate-Conductance Calcium-Activated Potassium Channels, DNA-Binding Proteins, Jurkat Cells, Mice, Phosphatidylinositol 3-Kinases, Mucoproteins, Proteolysis, Animals, Cytokines, Humans, Calcium, Polyubiquitin, Ion Channel Gating, Phosphoinositide-3 Kinase Inhibitors, Protein Binding, Signal Transduction
CD4-Positive T-Lymphocytes, Receptors, Antigen, T-Cell, Nuclear Proteins, Th1 Cells, Intermediate-Conductance Calcium-Activated Potassium Channels, DNA-Binding Proteins, Jurkat Cells, Mice, Phosphatidylinositol 3-Kinases, Mucoproteins, Proteolysis, Animals, Cytokines, Humans, Calcium, Polyubiquitin, Ion Channel Gating, Phosphoinositide-3 Kinase Inhibitors, Protein Binding, Signal Transduction
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