descriptionPublicationkeyboard_double_arrow_right Article 04 Dec 2002 English Publisher:WileyJournal:FEBS Letters, volume 533, pages 67-71 (issn: 0014-5793, eissn: 1873-3468,

Authors: Boutet-Robinet, Elisa A.; Finana, Frederic; Wurch, Thierry; Pauwels, Petrus J.; De Vries, Luc;

doi: 10.1016/s0014-5793(02)03753-5

pmid: 12505161

Endogenous RGS proteins facilitate dopamine D2S receptor coupling to Gαo proteins and Ca2+ responses in CHO‐K1 cells

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Abstract

The role of RGS proteins on dopaminergic D2S receptor (D2SR) signalling was investigated in Chinese hamster ovary (CHO)‐K1 cells, using recombinant RGS protein‐ and PTX‐insensitive Gαo proteins. Dopamine‐mediated [35S]GTPγS binding was attenuated by more than 60% in CHO‐K1 D2SR cells coexpressing a RGS protein‐ and PTX‐insensitive GαoGly184Ser:Cys351Ile protein versus cells coexpressing a similar amount of PTX‐insensitive GαoCys351Ile protein. Dopamine‐agonist‐mediated Ca2+ responses were dependent on the coexpression with a GαoCys351Ile protein and were fully abolished upon coexpression with a GαoGly184Ser:Cys351Ile protein. These results suggest that interactions between the Gαo protein and RGS proteins are involved in efficient D2SR signalling.

Keywords

Apomorphine, GTPγS, Receptors, Dopamine D2, Dopamine, CHO Cells, GTP-Binding Protein alpha Subunits, Gi-Go, Dopamine D2 receptor, Heterotrimeric GTP-Binding Proteins, Recombinant Proteins, RGS, Pertussis Toxin, G protein coupling, Cricetinae, Animals, Humans, Calcium Signaling, RGS Proteins, Ca2+ response, Signal Transduction

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