Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2
Copyright policy )Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2
Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.
- Yangzhou University China (People's Republic of)
DNA, Complementary, STAT2 Transcription Factor, Transfection, Protein Inhibitors of Activated STAT, Mice, HEK293 Cells, Two-Hybrid System Techniques, Protein Interaction Mapping, Animals, Humans, DNA Polymerase III, HeLa Cells
DNA, Complementary, STAT2 Transcription Factor, Transfection, Protein Inhibitors of Activated STAT, Mice, HEK293 Cells, Two-Hybrid System Techniques, Protein Interaction Mapping, Animals, Humans, DNA Polymerase III, HeLa Cells
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