Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice
pmid: 12869764
Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice
We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis . Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.
DNA, Complementary, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, Computational Biology, Oryza, Sequence Analysis, DNA, Genes, Plant, Protein Structure, Tertiary, Alternative Splicing, Open Reading Frames, Sequence Homology, Nucleic Acid, RNA, Antisense, Amino Acid Sequence, Cloning, Molecular, Databases, Nucleic Acid, Databases, Protein, Genome, Plant, Plant Proteins, Transcription Factors
DNA, Complementary, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, Computational Biology, Oryza, Sequence Analysis, DNA, Genes, Plant, Protein Structure, Tertiary, Alternative Splicing, Open Reading Frames, Sequence Homology, Nucleic Acid, RNA, Antisense, Amino Acid Sequence, Cloning, Molecular, Databases, Nucleic Acid, Databases, Protein, Genome, Plant, Plant Proteins, Transcription Factors
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