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Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice

Authors: Shoshi, Kikuchi; Kouji, Satoh; Toshifumi, Nagata; Nobuyuki, Kawagashira; Koji, Doi; Naoki, Kishimoto; Junshi, Yazaki; +67 Authors

Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice

Abstract

We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis . Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.

Keywords

DNA, Complementary, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, Computational Biology, Oryza, Sequence Analysis, DNA, Genes, Plant, Protein Structure, Tertiary, Alternative Splicing, Open Reading Frames, Sequence Homology, Nucleic Acid, RNA, Antisense, Amino Acid Sequence, Cloning, Molecular, Databases, Nucleic Acid, Databases, Protein, Genome, Plant, Plant Proteins, Transcription Factors

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
794
Top 1%
Top 0.1%
Top 0.1%