A Sensitive Enzyme Immunoassay for the Quantitation of Human C5a/C5a(desArg) Anaphylatoxin Using a Monoclonal Antibody with Specificity for a Neoepitope
Copyright policy )A Sensitive Enzyme Immunoassay for the Quantitation of Human C5a/C5a(desArg) Anaphylatoxin Using a Monoclonal Antibody with Specificity for a Neoepitope
The direct quantitation of C5a/C5a(desArg) in human plasma was achieved by a specific and highly sensitive ELISA which is based on the neoepitope-specific monoclonal antibody C17/5. The error-prone removal of C5 from plasma prior to the assay is therefore not required. With a detection limit of 20 pg C5a/ml plasma, the sensitivity of this assay allowed to define normal ranges (1.94 +/- 1.49 ng C5a/nl, mean +/- SD) of C5a/C5a(desArg) in human blood plasma. This assay will also be applicable to the quantitation of C5a in specimens with low protein content where precipitation-based assays fail to accurately determine C5a. In addition, the mAb C17/5 turned out to efficiently block the binding of C5a/C5a (desArg) to its cellular receptor and therefore provides a valuable tool in the delineation of C5a effects in complex biologic systems in the presence of native C5, such as under in vivo conditions.
- University of Göttingen Germany
Blood Platelets, Complement C5a, des-Arginine, Guinea Pigs, Temperature, Antibodies, Monoclonal, Blood Donors, Complement C5a, Enzyme-Linked Immunosorbent Assay, In Vitro Techniques, Sensitivity and Specificity, Epitopes, Adenosine Triphosphate, Antibody Specificity, Blood Preservation, Calibration, Animals, Humans
Blood Platelets, Complement C5a, des-Arginine, Guinea Pigs, Temperature, Antibodies, Monoclonal, Blood Donors, Complement C5a, Enzyme-Linked Immunosorbent Assay, In Vitro Techniques, Sensitivity and Specificity, Epitopes, Adenosine Triphosphate, Antibody Specificity, Blood Preservation, Calibration, Animals, Humans
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