Crystal Structure of β-Helical Antifreeze Protein Points to a General Ice Binding Model
Copyright policy )Crystal Structure of β-Helical Antifreeze Protein Points to a General Ice Binding Model
Reported here is the 2.3 A resolution crystal structure of spruce budworm (Choristoneura fumiferana) antifreeze protein (CfAFP), solved by single anomalous scattering. The structure reveals an extremely regular left-handed beta-helical platform consisting of 15-amino acid loops with a repetitive Thr-X-Thr motif displayed on one of the helix's three faces. This motif results in a two-dimensional array of threonine residues in an identical orientation to those in the nonhomologous, right-handed beta-helical beetle AFP from Tenebrio molitor (TmAFP). The CfAFP structure led us to reevaluate our ice binding model, and the analysis of three possible modes of docking gives rise to a binding mechanism based on surface complementarity. This general mechanism is applicable to both fish and insect AFPs.
- Queen's University Canada
Models, Molecular, Threonine, Magnetic Resonance Spectroscopy, Molecular Structure, surface complementarity, Ice, Fishes, Hydrogen Bonding, Moths, single anomalous scattering, Crystallography, X-Ray, Protein Structure, Secondary, Structural Biology, Antifreeze Proteins, Animals, β helix, X-ray structure, ice binding, antifreeze protein, Molecular Biology, Dimerization, Protein Binding
Models, Molecular, Threonine, Magnetic Resonance Spectroscopy, Molecular Structure, surface complementarity, Ice, Fishes, Hydrogen Bonding, Moths, single anomalous scattering, Crystallography, X-Ray, Protein Structure, Secondary, Structural Biology, Antifreeze Proteins, Animals, β helix, X-ray structure, ice binding, antifreeze protein, Molecular Biology, Dimerization, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).101 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!