Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA
Copyright policy )Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA
Abstract We report a series of synthetic, nucleic acid mimics with highly customizable thermodynamic binding to DNA. Incorporation of helix-promoting cyclopentanes into peptide nucleic acids (PNAs) increases the melting temperatures (Tm) of PNA+DNA duplexes by approximately +5°C per cyclopentane. Sequential addition of cyclopentanes allows the Tm of PNA + DNA duplexes to be systematically fine-tuned from +5 to +50°C compared with the unmodified PNA. Containing only nine nucleobases and an equal number of cyclopentanes, cpPNA-9 binds to complementary DNA with a Tm around 90°C. Additional experiments reveal that the cpPNA-9 sequence specifically binds to DNA duplexes containing its complementary sequence and functions as a PCR clamp. An X-ray crystal structure of the cpPNA-9–DNA duplex revealed that cyclopentanes likely induce a right-handed helix in the PNA with conformations that promote DNA binding.
- National Cancer Institute United States
- National Institutes of Health United States
- National Institute of Health Pakistan
- Department of Health and Human Services United States
- Austin Health & Human Services Department United States
Models, Molecular, Peptide Nucleic Acids, Circular Dichroism, Cyclopentanes, DNA, Calorimetry, Crystallography, X-Ray, Nucleic Acid Denaturation, Real-Time Polymerase Chain Reaction, Nucleic Acid Conformation, Thermodynamics, Transition Temperature, Spectrophotometry, Ultraviolet
Models, Molecular, Peptide Nucleic Acids, Circular Dichroism, Cyclopentanes, DNA, Calorimetry, Crystallography, X-Ray, Nucleic Acid Denaturation, Real-Time Polymerase Chain Reaction, Nucleic Acid Conformation, Thermodynamics, Transition Temperature, Spectrophotometry, Ultraviolet
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).28 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!