Secretome-Based Identification of Mac-2 Binding Protein as a Potential Oral Cancer Marker Involved in Cell Growth and Motility
Copyright policy )Secretome-Based Identification of Mac-2 Binding Protein as a Potential Oral Cancer Marker Involved in Cell Growth and Motility
Oral squamous cell carcinoma (OSCC) is the 11th most common cancer worldwide, and is associated with a high death rate. At present, there are no suitable markers for detecting and/or monitoring OSCC in body fluids/tissues. Here, we used 1D SDS-PAGE and MALDI-TOF MS to systematically analyze the secretomes of two OSCC cell lines (OEC-M1 and SCC4). The putative OSCC-related proteins identified in this analysis included the Mac-2 binding protein (Mac-2 BP), which was further found to be overexpressed in OSCC specimens and significantly elevated in the sera of OSCC patients compared to healthy controls. Finally, RNA interference-based knock-down of Mac-2 BP expression in OSCC cells revealed for the first time that Mac-2 BP is involved in regulating growth and motility of OSCC cells.
- Chang Gung Memorial Hospital Taiwan
- Chang Gung University Taiwan
Adult, Male, Membrane Glycoproteins, Base Sequence, Proteome, Cell Cycle, Enzyme-Linked Immunosorbent Assay, Middle Aged, Immunohistochemistry, Antigens, Neoplasm, Cell Movement, Culture Media, Conditioned, Biomarkers, Tumor, Carcinoma, Squamous Cell, Humans, Electrophoresis, Polyacrylamide Gel, Female, Mouth Neoplasms, Cell Division, Aged
Adult, Male, Membrane Glycoproteins, Base Sequence, Proteome, Cell Cycle, Enzyme-Linked Immunosorbent Assay, Middle Aged, Immunohistochemistry, Antigens, Neoplasm, Cell Movement, Culture Media, Conditioned, Biomarkers, Tumor, Carcinoma, Squamous Cell, Humans, Electrophoresis, Polyacrylamide Gel, Female, Mouth Neoplasms, Cell Division, Aged
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