Coactivator and Promoter-Selective Properties of RNA Polymerase I TAFs
Copyright policy )Coactivator and Promoter-Selective Properties of RNA Polymerase I TAFs
Human ribosomal RNA synthesis by RNA polymerase I requires the activator UBF and the promoter selectivity factor SL1, which consists of the TATA binding protein (TBP) and three associated subunits, TAF I 110, TAF I 63, and TAF I 48. Here it is shown that both TAF I 110 and TAF I 63 contact the promoter, whereas TAF I 48 serves as a target for interaction with UBF and is required to form a transcriptionally active SL1 complex responsive to UBF in vitro. TAF I 48 also alters the ability of TBP to interact with TATA box elements, and the resulting complex fails to support transcription by RNA polymerase II. Thus, TAF I 48 may function both as a target to mediate UBF activation and as a class-specific promoter selectivity factor.
- Howard Hughes Medical Institute United States
- University of California, Berkeley United States
TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, TATA Box, Recombinant Proteins, DNA-Binding Proteins, RNA Polymerase I, RNA, Ribosomal, Humans, Transcription Factor TFIID, RNA Polymerase II, Promoter Regions, Genetic, Pol1 Transcription Initiation Complex Proteins, HeLa Cells, Transcription Factors
TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, TATA Box, Recombinant Proteins, DNA-Binding Proteins, RNA Polymerase I, RNA, Ribosomal, Humans, Transcription Factor TFIID, RNA Polymerase II, Promoter Regions, Genetic, Pol1 Transcription Initiation Complex Proteins, HeLa Cells, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).96 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!