Identification of a NovelLy49Promoter That Is Active in Bone Marrow and Fetal Thymus
Copyright policy )Identification of a NovelLy49Promoter That Is Active in Bone Marrow and Fetal Thymus
AbstractThe analysis of several Ly49 genes has identified a tissue-specific promoter adjacent to the previously defined first exon. The current study reveals the presence of an additional Ly49 promoter (Pro-1) and two noncoding exons upstream of the previously defined promoter (Pro-2). DNA sequences homologous to Pro-1 are present 4–10 kb upstream of Pro-2 in all Ly49 genes examined, and Pro-1 transcripts were detected from the Ly49a, e, g, o, and v genes. Pro-1 activity can be detected in bone marrow, embryonic thymus, freshly isolated liver NK cells, and the murine LNK cell line, but it does not function in adult thymus, sorted NK-T cells, spleen NK cells, or the EL-4 T cell line, even though these cells express Ly49 proteins. Luciferase reporter assays identified a Pro-1 core promoter region that functions in the LNK cell line but not EL-4 cells. The novel promoter is not active in mature NK cells, suggesting that Pro-1 represents an early Ly49 promoter.
- National Cancer Institute United States
- National Cancer Institute Ukraine
- Center for Cancer Research United States
- Science Applications International Corporation (United States) United States
Mice, Inbred BALB C, Membrane Glycoproteins, Base Sequence, Molecular Sequence Data, Bone Marrow Cells, Exons, Cell Line, Killer Cells, Natural, Mice, Inbred C57BL, Alternative Splicing, Mice, Fetus, Gene Expression Regulation, Liver, Multigene Family, Animals, Antigens, Ly, Lectins, C-Type, 5' Untranslated Regions, NK Cell Lectin-Like Receptor Subfamily A
Mice, Inbred BALB C, Membrane Glycoproteins, Base Sequence, Molecular Sequence Data, Bone Marrow Cells, Exons, Cell Line, Killer Cells, Natural, Mice, Inbred C57BL, Alternative Splicing, Mice, Fetus, Gene Expression Regulation, Liver, Multigene Family, Animals, Antigens, Ly, Lectins, C-Type, 5' Untranslated Regions, NK Cell Lectin-Like Receptor Subfamily A
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