Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals
Copyright policy )Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals
Pervasive transcription initiates from cryptic promoters and is observed in eukaryotes ranging from yeast to mammals. The Set2-Rpd3 regulatory system prevents cryptic promoter function within expressed genes. However, conserved systems that control pervasive transcription within intergenic regions have not been well established. Here we show that Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae and murine embryonic stem cells (mESCs). In yeast, all three proteins bind subtelomeric heterochromatin through a Sir3-stimulated mechanism and to euchromatin via a TBP-stimulated mechanism. In mESCs, the proteins bind to active and poised TBP-bound promoters along with promoters of polycomb-silenced genes apparently lacking TBP. Depletion of Mot1, Ino80C, or NC2 by anchor away in yeast or RNAi in mESCs leads to near-identical transcriptome phenotypes, with new subtelomeric transcription in yeast, and greatly increased pervasive transcription in both yeast and mESCs.
- University of California, Los Angeles United States
- University of Massachusetts Medical School United States
- Utrecht University Netherlands
- University Medical Center Utrecht Netherlands
- University of California, San Francisco United States
Biomedical and clinical sciences, Stem Cell Research - Embryonic - Non-Human, Medical and Health Sciences, NC2, Euchromatin, Silent Information Regulator Proteins, Gene Expression Regulation, Fungal, Heterochromatin, Promoter Regions, Genetic, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Adenosine Triphosphatases, pervasive transcription, Biological Sciences, DNA-Binding Proteins, Biological sciences, Fungal, Phenotype, RNA Interference, Transcription, Protein Binding, Saccharomyces cerevisiae Proteins, Genotype, 1.1 Normal biological development and functioning, Bioinformatics and Computational Biology, Saccharomyces cerevisiae, Transfection, Cell Line, Promoter Regions, Genetic, 616, Genetics, Gene Silencing, Embryonic Stem Cells, TATA-Binding Protein Associated Factors, promoter, Binding Sites, Human Genome, heterochromatin, 500, Health sciences, Stem Cell Research, TATA-Box Binding Protein, Phosphoproteins, Repressor Proteins, Gene Expression Regulation, silencing, ATPases Associated with Diverse Cellular Activities, Transcription Factor TFIID, Sir3, Biochemistry and Cell Biology, Generic health relevance, polycomb, Mot1, Transcription Factors, Ino80, Developmental Biology
Biomedical and clinical sciences, Stem Cell Research - Embryonic - Non-Human, Medical and Health Sciences, NC2, Euchromatin, Silent Information Regulator Proteins, Gene Expression Regulation, Fungal, Heterochromatin, Promoter Regions, Genetic, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Adenosine Triphosphatases, pervasive transcription, Biological Sciences, DNA-Binding Proteins, Biological sciences, Fungal, Phenotype, RNA Interference, Transcription, Protein Binding, Saccharomyces cerevisiae Proteins, Genotype, 1.1 Normal biological development and functioning, Bioinformatics and Computational Biology, Saccharomyces cerevisiae, Transfection, Cell Line, Promoter Regions, Genetic, 616, Genetics, Gene Silencing, Embryonic Stem Cells, TATA-Binding Protein Associated Factors, promoter, Binding Sites, Human Genome, heterochromatin, 500, Health sciences, Stem Cell Research, TATA-Box Binding Protein, Phosphoproteins, Repressor Proteins, Gene Expression Regulation, silencing, ATPases Associated with Diverse Cellular Activities, Transcription Factor TFIID, Sir3, Biochemistry and Cell Biology, Generic health relevance, polycomb, Mot1, Transcription Factors, Ino80, Developmental Biology
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