Honokiol inhibits HepG2 migration via down‐regulation of IQGAP1 expression discovered by a quantitative pharmaceutical proteomic analysis
Copyright policy )Honokiol inhibits HepG2 migration via down‐regulation of IQGAP1 expression discovered by a quantitative pharmaceutical proteomic analysis
AbstractHonokiol (HNK), a natural small molecular product, inhibited proliferation of HepG2 cells and exhibited anti‐tumor activity in nude mice. In this article, we applied a novel sensitive stable isotope labeling with amino acids in cell culture‐based quantitative proteomic method and a model of nude mice to investigate the correlation between HNK and the hotspot migration molecule Ras GTPase‐activating‐like protein (IQGAP1). The quantitative proteomic analysis showed that IQGAP1 was 0.53‐fold down‐regulated under 10 μg/mL HNK exposure for 24 h on HepG2 cells. Migration ability of HepG2 cells under HNK treatment was correlated with its expression level of IQGAP1. In addition, the biochemical validation on HepG2 cells and the tumor xenograft model further demonstrated that HNK decreased the expression level of IQGAP1 and its upstream proteins Cdc42/Rac1. These data supported that HNK can modulate cell adhesion and cell migration by acting on Cdc42/Rac1 signaling via IQGAP1 interactions with its upstream Cdc42/Rac1 proteins, which is a new molecular mechanism of HNK to exert its anti‐tumor activity.
- Sichuan University China (People's Republic of)
Proteomics, rac1 GTP-Binding Protein, Proteome, Histocytochemistry, Biphenyl Compounds, Down-Regulation, Mice, Nude, Reproducibility of Results, Hep G2 Cells, Antineoplastic Agents, Phytogenic, Lignans, Statistics, Nonparametric, Tumor Burden, Mice, Cell Movement, ras GTPase-Activating Proteins, Isotope Labeling, Animals, Humans, cdc42 GTP-Binding Protein
Proteomics, rac1 GTP-Binding Protein, Proteome, Histocytochemistry, Biphenyl Compounds, Down-Regulation, Mice, Nude, Reproducibility of Results, Hep G2 Cells, Antineoplastic Agents, Phytogenic, Lignans, Statistics, Nonparametric, Tumor Burden, Mice, Cell Movement, ras GTPase-Activating Proteins, Isotope Labeling, Animals, Humans, cdc42 GTP-Binding Protein
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