Insulin-Like Growth Factor Binding Protein-3 in Extracellular Matrix Stimulates Adhesion of Breast Epithelial Cells and Activation of p44/42 Mitogen-Activated Protein Kinase
Copyright policy )Insulin-Like Growth Factor Binding Protein-3 in Extracellular Matrix Stimulates Adhesion of Breast Epithelial Cells and Activation of p44/42 Mitogen-Activated Protein Kinase
IGF-binding protein-3 (IGFBP-3) is a multifunctional protein that regulates the potent mitogenic and antiapoptotic effects of IGF-I and IGF-II and exerts bioactivity independent of modulating IGF receptor activation. Previous studies have shown that in solution, IGFBP-3 binds constituent proteins of the extracellular matrix (ECM) such as fibronectin and collagen and is present in ECM deposited by fibroblasts in vitro; however, binding of IGFBP-3 to matrix has not been characterized, nor has its function in this environment been investigated. In this study, we show that IGFBP-3 binds to ECM deposited by human breast epithelial and cancer cells and neonatal human fibroblasts. IGF-I and heparin blocked binding of IGFBP-3 to matrix when added with the binding protein but were unable to displace IGFBP-3 already bound to the matrix. IGF-I bound to matrix-immobilized IGFBP-3 with approximately 25-fold reduced affinity compared with IGFBP-3 in solution. Mutation of the C-terminal basic domain of IGFBP-3 (228KGRKR→MDGEA) resulted in markedly reduced binding to matrix compared with wild-type IGFBP-3, whereas mutation of the adjacent consensus heparin-binding domain (220KKK→HSR) had relatively little effect. In the presence of matrix-bound IGFBP-3, adhesion of breast epithelial cells was increased by approximately 25%, and activation of the signaling pathway intermediate p44/42 MAPK was enhanced greater than 3-fold. These results indicate a previously unrecognized and potentially important role for IGFBP-3 in the extracellular matrix.
- University of Sydney Australia
- Kolling Institute of Medical Research Australia
- Royal North Shore Hospital Australia
Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Heparin, Breast Neoplasms, Epithelial Cells, Extracellular Matrix, Enzyme Activation, Solutions, Insulin-Like Growth Factor Binding Protein 3, Cell Line, Tumor, Cell Adhesion, Humans, Breast, Insulin-Like Growth Factor I, Cells, Cultured, Signal Transduction
Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Heparin, Breast Neoplasms, Epithelial Cells, Extracellular Matrix, Enzyme Activation, Solutions, Insulin-Like Growth Factor Binding Protein 3, Cell Line, Tumor, Cell Adhesion, Humans, Breast, Insulin-Like Growth Factor I, Cells, Cultured, Signal Transduction
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