Characterization of the Mouse Adeno-Associated Virus AAVS1 Ortholog
Copyright policy )Characterization of the Mouse Adeno-Associated Virus AAVS1 Ortholog
ABSTRACT The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1 ), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proximal to the recently identified Mbs85 gene. These sequence motifs can be specifically nicked by the viral Rep protein required for the initiation of site-specific AAV DNA integration. Furthermore, these signals can serve as a minimal origin for Rep-dependent DNA replication. In addition, we isolated the mouse Mbs85 proximal promoter and show transcriptional activity in three mouse cell lines.
- King's College London United Kingdom
- KU Leuven Belgium
- Kings College London, University of London United Kingdom
- Icahn School of Medicine at Mount Sinai United States
- University of Florida United States
570, TROPONIN-I, Virus Integration, Molecular Sequence Data, SITE-SPECIFIC INTEGRATION, 610, Cell Line, 30 Agricultural, veterinary and food sciences, Mice, Virology, 07 Agricultural and Veterinary Sciences, DNA HELICASE ACTIVITY, REP PROTEINS, Animals, Humans, 11 Medical and Health Sciences, Science & Technology, Base Sequence, TARGETED INTEGRATION, HUMAN-CHROMOSOME 19, 31 Biological sciences, Proteins, 32 Biomedical and clinical sciences, 06 Biological Sciences, Dependovirus, SEQUENCE REQUIREMENTS, BINDING-PROTEIN, Hela Cells, REPLICATION IN-VITRO, NIH 3T3 Cells, Life Sciences & Biomedicine, Chromosomes, Human, Pair 19, Sequence Alignment, TERMINAL REPEATS, HeLa Cells
570, TROPONIN-I, Virus Integration, Molecular Sequence Data, SITE-SPECIFIC INTEGRATION, 610, Cell Line, 30 Agricultural, veterinary and food sciences, Mice, Virology, 07 Agricultural and Veterinary Sciences, DNA HELICASE ACTIVITY, REP PROTEINS, Animals, Humans, 11 Medical and Health Sciences, Science & Technology, Base Sequence, TARGETED INTEGRATION, HUMAN-CHROMOSOME 19, 31 Biological sciences, Proteins, 32 Biomedical and clinical sciences, 06 Biological Sciences, Dependovirus, SEQUENCE REQUIREMENTS, BINDING-PROTEIN, Hela Cells, REPLICATION IN-VITRO, NIH 3T3 Cells, Life Sciences & Biomedicine, Chromosomes, Human, Pair 19, Sequence Alignment, TERMINAL REPEATS, HeLa Cells
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