Enhancement of the Specific Mucosal Iga Response In Vivo by Interleukin-5 Expressed by an Attenuated Strain of Salmonella Serotype Dublin
Copyright policy )Enhancement of the Specific Mucosal Iga Response In Vivo by Interleukin-5 Expressed by an Attenuated Strain of Salmonella Serotype Dublin
It has been shown that cytokines have potential as therapeutic adjuvants in vaccination. Interleukin-5 (IL-5) is a cytokine that regulates antibody production, in particular enhancing IgA production by activated mucosal B cells. This study examined the expression of a cloned cytokine gene encoding murine IL-5 (mIL-5) by an attenuated aroA strain (SL5631) of Salmonella serotype Dublin. The resulting strain, SL5631(pTRXFUS-mIL-5), expressed mIL-5 as a fusion with thioredoxin as demonstrated by immunological and biological assays. When strain SL5631(pTRXFUS-mIL-5) was used as a live vaccine in BALB/c mice, it colonised and multiplied at higher levels in spleens and livers than the strain carrying the empty plasmid. A reduction in invasiveness of SL5631(pTRXFUS-mIL-5) was observed in in-vitro invasion assays. Enhanced IgA response against salmonella LPS in mucosal secretions and enhanced IgA and IgG responses were detected by ELISA and ELISPOT methods in sera of mice immunised with the strain expressing mIL-5. Results with IL-5-deficient mice showed that the enhanced IgA response was due to Salmonella-expressed mIL-5 rather than endogenous mIL-5.
Lipopolysaccharides, Mice, Inbred BALB C, Genetic Vectors, Gene Expression, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Mice, Inbred C57BL, Disease Models, Animal, Mice, Salmonella, Immunoglobulin G, Bacterial Vaccines, Immunoglobulin A, Secretory, Animals, Electrophoresis, Polyacrylamide Gel, Female, Cloning, Molecular, Interleukin-5, Immunity, Mucosal, Plasmids
Lipopolysaccharides, Mice, Inbred BALB C, Genetic Vectors, Gene Expression, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Mice, Inbred C57BL, Disease Models, Animal, Mice, Salmonella, Immunoglobulin G, Bacterial Vaccines, Immunoglobulin A, Secretory, Animals, Electrophoresis, Polyacrylamide Gel, Female, Cloning, Molecular, Interleukin-5, Immunity, Mucosal, Plasmids
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).17 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!