A requirement for replication in activation of the ATR-dependent DNA damage checkpoint
Copyright policy )A requirement for replication in activation of the ATR-dependent DNA damage checkpoint
Using the Xenopus egg extract system, we investigated the involvement of DNA replication in activation of the DNA damage checkpoint. We show here that DNA damage slows replication in a checkpoint-independent manner and is accompanied by replication-dependent recruitment of ATR and Rad1 to chromatin. We also find that the replication proteins RPA and Polα accumulate on chromatin following DNA damage. Finally, damage-induced Chk1 phosphorylation and checkpoint arrest are abrogated when replication is inhibited. These data indicate that replication is required for activation of the DNA damage checkpoint and suggest a unifying model for ATR activation by diverse lesions during S phase.
- Stanford University United States
DNA Replication, Ultraviolet Rays, Xenopus, Cell Cycle, Cell Cycle Proteins, Ataxia Telangiectasia Mutated Proteins, In Vitro Techniques, Protein Serine-Threonine Kinases, Xenopus Proteins, Endonucleases, Methyl Methanesulfonate, Models, Biological, Recombinant Proteins, DNA-Binding Proteins, Checkpoint Kinase 1, Oocytes, Animals, Female, Protein Kinases, DNA Damage
DNA Replication, Ultraviolet Rays, Xenopus, Cell Cycle, Cell Cycle Proteins, Ataxia Telangiectasia Mutated Proteins, In Vitro Techniques, Protein Serine-Threonine Kinases, Xenopus Proteins, Endonucleases, Methyl Methanesulfonate, Models, Biological, Recombinant Proteins, DNA-Binding Proteins, Checkpoint Kinase 1, Oocytes, Animals, Female, Protein Kinases, DNA Damage
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