MOV10 and FMRP Regulate AGO2 Association with MicroRNA Recognition Elements
Copyright policy )MOV10 and FMRP Regulate AGO2 Association with MicroRNA Recognition Elements
The fragile X mental retardation protein FMRP regulates translation of its bound mRNAs through incompletely defined mechanisms. FMRP has been linked to the microRNA pathway, and we show here that it associates with the RNA helicase MOV10, also associated with the microRNA pathway. FMRP associates with MOV10 directly and in an RNA-dependent manner and facilitates MOV10's association with RNAs in brain and cells, suggesting a cooperative interaction. We identified the RNAs recognized by MOV10 using RNA immunoprecipitation and iCLIP. Examination of the fate of MOV10 on RNAs revealed a dual function for MOV10 in regulating translation: it facilitates microRNA-mediated translation of some RNAs, but it also increases expression of other RNAs by preventing AGO2 function. The latter subset was also bound by FMRP in close proximity to the MOV10 binding site, suggesting that FMRP prevents MOV10-mediated microRNA suppression. We have identified a mechanism for FMRP-mediated translational regulation through its association with MOV10.
- University of California, Santa Barbara United States
- University of Illinois at Urbana Champaign United States
- Molecular Research Institute United States
- University of California, San Francisco United States
- University of Illinois at Urbana–Champaign United States
QH301-705.5, Intellectual and Developmental Disabilities (IDD), 1.1 Normal biological development and functioning, Knockout, Medical Physiology, Fragile X Mental Retardation Protein, Mice, Rare Diseases, Underpinning research, Genetics, 2.1 Biological and endogenous factors, Animals, Humans, Aetiology, Biology (General), 3' Untranslated Regions, Mice, Knockout, Binding Sites, Brain, Biological Sciences, Brain Disorders, GC Rich Sequence, Biological sciences, MicroRNAs, HEK293 Cells, Fragile X Syndrome, Argonaute Proteins, RNA Interference, Biochemistry and Cell Biology, Transcriptome, RNA Helicases, Biotechnology, Protein Binding
QH301-705.5, Intellectual and Developmental Disabilities (IDD), 1.1 Normal biological development and functioning, Knockout, Medical Physiology, Fragile X Mental Retardation Protein, Mice, Rare Diseases, Underpinning research, Genetics, 2.1 Biological and endogenous factors, Animals, Humans, Aetiology, Biology (General), 3' Untranslated Regions, Mice, Knockout, Binding Sites, Brain, Biological Sciences, Brain Disorders, GC Rich Sequence, Biological sciences, MicroRNAs, HEK293 Cells, Fragile X Syndrome, Argonaute Proteins, RNA Interference, Biochemistry and Cell Biology, Transcriptome, RNA Helicases, Biotechnology, Protein Binding
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