Minor Complexes at Work: Light-Harvesting by Carotenoids in the Photosystem II Antenna Complexes CP24 and CP26
pmid: 21641329
pmc: PMC3117163
Minor Complexes at Work: Light-Harvesting by Carotenoids in the Photosystem II Antenna Complexes CP24 and CP26
Plant photosynthesis relies on the capacity of chlorophylls and carotenoids to absorb light. One of the roles of carotenoids is to harvest green-blue light and transfer the excitation energy to the chlorophylls. The corresponding dynamics were investigated here for the first time, to our knowledge, in the CP26 and CP24 minor antenna complexes. The results for the two complexes differ substantially. In CP26 fast transfer (80 fs) occurs from the carotenoid S(2) state to chlorophylls a absorbing at 675 and 678 nm, whereas transfer from the hot S(1) state to the lowest energy chlorophylls is observed in <1 ps. In CP24, energy transfer from the S(2) state leads in 80 fs to the population of chlorophylls b and high-energy chlorophylls a absorbing at 670 nm, whereas the low-energy chlorophylls a are populated only in several picoseconds. The results suggest that CP26 has a structural and functional organization similar to that of LHCII, whereas CP24 differs substantially from the other Lhc complexes, especially regarding the lutein L1 binding domain. No energy transfer from the carotenoid S(1) state to chlorophylls was observed in either complex, suggesting that this state is energetically below the chlorophyll Qy state and therefore may play a role in the quenching of chlorophyll excitations.
- Vrije Universiteit Amsterdam Netherlands
- University of Groningen Netherlands
- University of Gronigen Netherlands
Chlorophyll, CHLOROPHYLL ENERGY-TRANSFER, S-1 STATE, Light, GREEN PLANTS, Biophysics, Arabidopsis, Light-Harvesting Protein Complexes, EXCITED-STATE, TRANSFER PATHWAYS, BINDING-SITES, HIGHER-PLANTS, Absorption, LOW-TEMPERATURE, SDG 7 - Affordable and Clean Energy, Arabidopsis Proteins, Spectrum Analysis, Photosystem II Protein Complex, FUNCTIONAL ARCHITECTURE, Carotenoids, Energy Transfer, Chlorophyll Binding Proteins, FEMTOSECOND TRANSIENT ABSORPTION, Protein Binding
Chlorophyll, CHLOROPHYLL ENERGY-TRANSFER, S-1 STATE, Light, GREEN PLANTS, Biophysics, Arabidopsis, Light-Harvesting Protein Complexes, EXCITED-STATE, TRANSFER PATHWAYS, BINDING-SITES, HIGHER-PLANTS, Absorption, LOW-TEMPERATURE, SDG 7 - Affordable and Clean Energy, Arabidopsis Proteins, Spectrum Analysis, Photosystem II Protein Complex, FUNCTIONAL ARCHITECTURE, Carotenoids, Energy Transfer, Chlorophyll Binding Proteins, FEMTOSECOND TRANSIENT ABSORPTION, Protein Binding
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