Association of the Kinesin Motor KIF1A with the Multimodular Protein Liprin-α
Copyright policy )Association of the Kinesin Motor KIF1A with the Multimodular Protein Liprin-α
Liprin-alpha/SYD-2 is a multimodular scaffolding protein important for presynaptic differentiation and postsynaptic targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid glutamate receptors. However, the molecular mechanisms underlying these functions remain largely unknown. Here we report that liprin-alpha interacts with the neuron-specific kinesin motor KIF1A. KIF1A colocalizes with liprin-alpha in various subcellular regions of neurons. KIF1A coaccumulates with liprin-alpha in ligated sciatic nerves. KIF1A cofractionates and coimmunopreciptates with liprin-alpha and various liprin-alpha-associated membrane, signaling, and scaffolding proteins including alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors, GRIP/ABP, RIM, GIT1, and beta PIX. These results suggest that liprin-alpha functions as a KIF1A receptor, linking KIF1A to various liprin-alpha-associated proteins for their transport in neurons.
- Dana-Farber Cancer Institute United States
- University of North Carolina at Chapel Hill United States
- Korea Advanced Institute of Science and Technology Korea (Republic of)
- Harvard University United States
- Korea Advanced Institute of Science and Technology Korea (Republic of)
Brain, Kinesins, Nerve Tissue Proteins, Phosphoproteins, Immunohistochemistry, Precipitin Tests, Rats, Two-Hybrid System Techniques, Animals, Receptors, AMPA, Microscopy, Immunoelectron, Adaptor Proteins, Signal Transducing, Protein Binding, Signal Transduction, Subcellular Fractions
Brain, Kinesins, Nerve Tissue Proteins, Phosphoproteins, Immunohistochemistry, Precipitin Tests, Rats, Two-Hybrid System Techniques, Animals, Receptors, AMPA, Microscopy, Immunoelectron, Adaptor Proteins, Signal Transducing, Protein Binding, Signal Transduction, Subcellular Fractions
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