Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates
Copyright policy )Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates
Timely removal of DNA recombination intermediates is critical for genome stability. The DNA helicase-topoisomerase complex, Sgs1-Top3-Rmi1 (STR), is the major pathway for processing these intermediates to generate conservative products. However, the mechanisms that promote STR-mediated functions remain to be defined. Here we show that Sgs1 binds to poly-SUMO chains and associates with the Smc5/6 SUMO E3 complex in yeast. Moreover, these interactions contribute to the sumoylation of Sgs1, Top3, and Rmi1 upon the generation of recombination structures. We show that reduced STR sumoylation leads to accumulation of recombination structures, and impaired growth in conditions when these structures arise frequently, highlighting the importance of STR sumoylation. Mechanistically, sumoylation promotes STR inter-subunit interactions and accumulation at DNA repair centers. These findings expand the roles of sumoylation and Smc5/6 in genome maintenance by demonstrating that they foster STR functions in the removal of recombination intermediates.
Cell Reports, 16 (2)
ISSN:2666-3864
ISSN:2211-1247
- École Polytechnique Fédérale de Lausanne EPFL Switzerland
- Yale University United States
- University of Toronto Canada
- ETH Zurich Switzerland
- School of Medicine Yale University United States
DNA Replication, Recombination, Genetic, Saccharomyces cerevisiae Proteins, Sgs1, RecQ Helicases, QH301-705.5, Protein sumoylation, SUMO-1 Protein, Sumoylation, Cell Cycle Proteins, Saccharomyces cerevisiae, Article, protein sumoylation, DNA-Binding Proteins, Recombination intermediates; Protein sumoylation; Smc5/6; Sgs1, Two-Hybrid System Techniques, recombination intermediates, Amino Acid Sequence, Biology (General), Smc5/6, sgs1, DNA, Fungal, smc5/6, Recombination intermediates
DNA Replication, Recombination, Genetic, Saccharomyces cerevisiae Proteins, Sgs1, RecQ Helicases, QH301-705.5, Protein sumoylation, SUMO-1 Protein, Sumoylation, Cell Cycle Proteins, Saccharomyces cerevisiae, Article, protein sumoylation, DNA-Binding Proteins, Recombination intermediates; Protein sumoylation; Smc5/6; Sgs1, Two-Hybrid System Techniques, recombination intermediates, Amino Acid Sequence, Biology (General), Smc5/6, sgs1, DNA, Fungal, smc5/6, Recombination intermediates
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).69 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!