Platelet Endothelial Cell Adhesion Molecule Deficiency or Blockade Significantly Reduces Leukocyte Emigration in a Majority of Mouse Strains
Copyright policy )Platelet Endothelial Cell Adhesion Molecule Deficiency or Blockade Significantly Reduces Leukocyte Emigration in a Majority of Mouse Strains
Abstract PECAM is a molecule used specifically during the diapedesis step when neutrophils and monocytes leave the blood compartment. Anti-PECAM reagents, such as Abs and soluble fusion proteins, block diapedesis both in vivo and in vitro. However, the PECAM knockout mouse in C57BL/6 strain has no serious defects in most models of inflammation. We show in this study that the same PECAM knockout backcrossed into the FVB/n strain clearly has reduced leukocyte emigration in two models of inflammation. Furthermore, we show that anti-PECAM reagents can block leukocyte emigration in several other wild-type strains of mice like FVB/n, SJL, and the outbred strain Swiss Webster. This clearly shows that the C57BL/6 strain is uniquely able to compensate for the loss of PECAM function. Murine models of inflammatory disease that have been studied using C57BL/6 mice should be re-evaluated using FVB/n or other mouse strains to determine whether PECAM plays a role in those models.
- Cornell University United States
- Colorado State University United States
Mice, Knockout, Croton Oil, Down-Regulation, Peritonitis, Dermatitis, Contact, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Disease Models, Animal, Mice, Species Specificity, Cell Movement, Thioglycolates, Cell Migration Inhibition, Leukocytes, Animals, Gene Silencing, Antibodies, Blocking, Crosses, Genetic
Mice, Knockout, Croton Oil, Down-Regulation, Peritonitis, Dermatitis, Contact, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Disease Models, Animal, Mice, Species Specificity, Cell Movement, Thioglycolates, Cell Migration Inhibition, Leukocytes, Animals, Gene Silencing, Antibodies, Blocking, Crosses, Genetic
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