A nuclear cap binding protein complex involved in pre-mRNA splicing
Copyright policy )A nuclear cap binding protein complex involved in pre-mRNA splicing
A cap-binding protein complex (CBC) present in the nuclei of HeLa cells has been characterized. Purified CBC consists of two previously identified proteins, CBP80 and CBP20. These proteins are shown to cofractionate to apparent homogeneity and to be coimmunoprecipitable with anti-CBP80 antibodies. Analysis of the inhibition of pre-mRNA splicing in vitro and in vivo by chemically modified analogs of the cap structure, and of the binding of these analogs to CBC in vitro, suggests a role for the complex in splicing. Extracts immunodepleted of CBC do not efficiently splice an adenoviral pre-mRNA owing to blockage of an early step in splicing complex formation. CBC may therefore play a role in pre-mRNA recognition.
- European Molecular Biology Laboratory Germany
- University of Warsaw Poland
Cell Extracts, Cell Nucleus, RNA Caps, RNA Splicing, Recombinant Fusion Proteins, Molecular Sequence Data, RNA-Binding Proteins, Sequence Analysis, DNA, RNA Cap Analogs, Xenopus laevis, Antibody Specificity, RNA Cap-Binding Proteins, RNA Precursors, Spliceosomes, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, HeLa Cells, Protein Binding
Cell Extracts, Cell Nucleus, RNA Caps, RNA Splicing, Recombinant Fusion Proteins, Molecular Sequence Data, RNA-Binding Proteins, Sequence Analysis, DNA, RNA Cap Analogs, Xenopus laevis, Antibody Specificity, RNA Cap-Binding Proteins, RNA Precursors, Spliceosomes, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, HeLa Cells, Protein Binding
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