Vascular endothelial growth factor enhances angiotensin II-induced aneurysm formation in apolipoprotein E-deficient mice
Copyright policy )Vascular endothelial growth factor enhances angiotensin II-induced aneurysm formation in apolipoprotein E-deficient mice
Abdominal aortic aneurysm (AAA) development is associated with increased angiogenesis and overexpression of vascular endothelial growth factor (VEGF). Inhibition of angiogenesis results in attenuation of experimental aneurysms. This study investigated the effects of recombinant human (rh)VEGF on experimental aneurysms.Apolipoprotein E-deficient (apoE(-/-)) mice were assigned to one of four groups: (1) normal saline infusion (sham), (2) angiotensin-II (AngII) infusion, (3) AngII infusion plus 100 microg daily rhVEGF for 14 days (AngII+14dVEGF), or (4) AngII infusion plus 100 microg daily rhVEGF for 21 days (AngII+21dVEGF). Aortic maximum diameter and cross-sectional area were determined by magnetic resonance imaging and microscopy. All mice were sacrificed at day 28.Aneurysms developed in all mice in the AngII+14dVEGF and AngII+21dVEGF groups by day 21 compared with 40% in the AngII group. Treatment with rhVEGF increased maximum aortic diameter (P < .002) and cross-sectional area of aneurysms (P < .005) at day 21. This effect was maintained at day 28 (P < .0005). Decreasing rhVEGF treatment from 21 to 14 days did not attenuate aneurysm formation. Treatment with rhVEGF upregulated matrix metalloproteinase 2 gene expression within the aortic wall (P < .0009).Treatment with rhVEGF intensified the formation of AngII-induced aneurysms. Further studies are needed to investigate if antiangiogenic therapy may be a valid medical therapy against aneurysm expansion or rupture.
- St George's Hospital United Kingdom
- St George's Hospital United Kingdom
- St George’s University Hospitals NHS Foundation Trust United Kingdom
- Midlands Partnership NHS Foundation Trust United Kingdom
- University of Leicester United Kingdom
Enzymologic, Vascular Endothelial Growth Factor A, Male, Time Factors, Knockout, Gene Expression Regulation, Enzymologic, Injections, Mice, Apolipoproteins E, Animals, Humans, Abdominal, Intraperitoneal, Aorta, Abdominal, Aorta, Infusion Pumps, Pathologic, Mice, Knockout, Animal, Angiotensin II, Body Weight, Infusion Pumps, Implantable, Dilatation, Recombinant Proteins, Aortic Aneurysm, Disease Models, Animal, Gene Expression Regulation, Disease Models, Disease Progression, Matrix Metalloproteinase 2, Surgery, Implantable, Cardiology and Cardiovascular Medicine, Injections, Intraperitoneal, Magnetic Resonance Angiography, Aortic Aneurysm, Abdominal, Dilatation, Pathologic
Enzymologic, Vascular Endothelial Growth Factor A, Male, Time Factors, Knockout, Gene Expression Regulation, Enzymologic, Injections, Mice, Apolipoproteins E, Animals, Humans, Abdominal, Intraperitoneal, Aorta, Abdominal, Aorta, Infusion Pumps, Pathologic, Mice, Knockout, Animal, Angiotensin II, Body Weight, Infusion Pumps, Implantable, Dilatation, Recombinant Proteins, Aortic Aneurysm, Disease Models, Animal, Gene Expression Regulation, Disease Models, Disease Progression, Matrix Metalloproteinase 2, Surgery, Implantable, Cardiology and Cardiovascular Medicine, Injections, Intraperitoneal, Magnetic Resonance Angiography, Aortic Aneurysm, Abdominal, Dilatation, Pathologic
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