Swapping of the β-Hairpin Region between Sp1 and GLI Zinc Fingers: Significant Role of the β-Hairpin Region in DNA Binding Properties of C2H2-type Zinc Finger Peptides
Copyright policy )Swapping of the β-Hairpin Region between Sp1 and GLI Zinc Fingers: Significant Role of the β-Hairpin Region in DNA Binding Properties of C2H2-type Zinc Finger Peptides
The recent design strategy of zinc finger peptides has mainly focused on the alpha-helix region, which plays a direct role in DNA recognition. On the other hand, the study of non-DNA-contacting regions is extremely scarce. By swapping the beta-hairpin regions between the Sp1 and GLI zinc fingers, in this study, we investigated how the beta-hairpin region of the C(2)H(2)-type zinc finger peptides contributes to the DNA binding properties. Surprisingly, the Sp1 mutant with the GLI-type beta-hairpin had a higher DNA binding affinity than that of the wild-type Sp1. The result of the DNase I footprinting analyses also showed the change in the DNA binding pattern. In contrast, the GLI zinc finger completely lost DNA binding ability as a result of exchanging the beta-hairpin region. These results strongly indicate that the beta-hairpin region appears to function as a scaffold and has an important effect on the DNA binding properties of the C(2)H(2)-type zinc finger peptides.
- Kyoto University Japan
Oncogene Proteins, Protein Conformation, Sp1 Transcription Factor, Circular Dichroism, Molecular Sequence Data, DNA Footprinting, Hydrogen Bonding, Zinc Fingers, DNA Methylation, Polymerase Chain Reaction, Zinc Finger Protein GLI1, Peptide Fragments, Protein Structure, Secondary, DNA-Binding Proteins, Trans-Activators, Deoxyribonuclease I, Humans, Amino Acid Sequence, Protein Binding, Transcription Factors
Oncogene Proteins, Protein Conformation, Sp1 Transcription Factor, Circular Dichroism, Molecular Sequence Data, DNA Footprinting, Hydrogen Bonding, Zinc Fingers, DNA Methylation, Polymerase Chain Reaction, Zinc Finger Protein GLI1, Peptide Fragments, Protein Structure, Secondary, DNA-Binding Proteins, Trans-Activators, Deoxyribonuclease I, Humans, Amino Acid Sequence, Protein Binding, Transcription Factors
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