A Cryptic DNA Binding Domain at the COOH Terminus of TFIIIB70 Affects Formation, Stability, and Function of Preinitiation Complexes
Copyright policy )A Cryptic DNA Binding Domain at the COOH Terminus of TFIIIB70 Affects Formation, Stability, and Function of Preinitiation Complexes
TFIIIC-dependent assembly of yeast TFIIIB on class III genes unmasks a high avidity of TFIIIB for DNA. TFIIIB contains TATA-binding protein (TBP), TFIIIB90/B", and TFIIIB70/Brf1, which is homologous to TFIIB. Using limited proteolysis, we have found that the COOH terminus of TFIIIB70 (residues 510-596) forms a protease-resistant domain that binds DNA tightly as seen by Southwestern, DNase I footprinting, and gel shift assays. Consistent with a role for this DNA binding activity, preinitiation complexes were formed less efficiently with truncated TFIIIB70 lacking the COOH-terminal domain and displayed an increased sensitivity to heparin. B' (TFIIIB70 + TBP).TFIIIC.DNA complexes were also particularly unstable. In addition, TFIIIB.TFIIIC.DNA complexes containing truncated TFIIIB70 were impaired in promoting transcription initiation.
TATA-Binding Protein Associated Factors, Binding Sites, Heparin, Molecular Sequence Data, DNA, Saccharomyces cerevisiae, TATA-Box Binding Protein, TATA Box, Peptide Fragments, Recombinant Proteins, DNA-Binding Proteins, Transcription Factor TFIIIB, Mutagenesis, Site-Directed, Animals, Humans, Amino Acid Sequence, Caenorhabditis elegans, Sequence Alignment, Conserved Sequence, Sequence Tagged Sites
TATA-Binding Protein Associated Factors, Binding Sites, Heparin, Molecular Sequence Data, DNA, Saccharomyces cerevisiae, TATA-Box Binding Protein, TATA Box, Peptide Fragments, Recombinant Proteins, DNA-Binding Proteins, Transcription Factor TFIIIB, Mutagenesis, Site-Directed, Animals, Humans, Amino Acid Sequence, Caenorhabditis elegans, Sequence Alignment, Conserved Sequence, Sequence Tagged Sites
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).27 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!