Global fitness profiling of fission yeast deletion strains by barcode sequencing
Copyright policy )Global fitness profiling of fission yeast deletion strains by barcode sequencing
AbstractA genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeastSchizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.
- National Institute of Biological Sciences, Beijing China (People's Republic of)
Electronic Data Processing, Base Sequence, Method, Reproducibility of Results, Sequence Analysis, DNA, Haploidy, Microtubules, Culture Media, Thiabendazole, Schizosaccharomyces, Cluster Analysis, Hydroxyurea, Camptothecin, Gene Silencing, Genetic Fitness, Gene Deletion, Mutagens
Electronic Data Processing, Base Sequence, Method, Reproducibility of Results, Sequence Analysis, DNA, Haploidy, Microtubules, Culture Media, Thiabendazole, Schizosaccharomyces, Cluster Analysis, Hydroxyurea, Camptothecin, Gene Silencing, Genetic Fitness, Gene Deletion, Mutagens
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