Human Granulosa-Lutein Cells Express Functional EP1 and EP2 Prostaglandin Receptors
Copyright policy )Human Granulosa-Lutein Cells Express Functional EP1 and EP2 Prostaglandin Receptors
Prostaglandin E(2) (PGE(2)) exerts mainly luteotrophic effects in the corpus luteum. In other tissues, PGE(2) acts via specific PGE(2) receptor subtypes including EP1, which modulates intracellular calcium ([Ca(2+)](i)) and EP2, which is coupled to cyclic AMP (cAMP) generation. We have therefore investigated the presence of functional EP1 and EP2 receptors using human granulosa-lutein (GL) cells. Reverse-transcription PCR revealed that GL cells expressed mRNA transcripts encoding both EP1 and EP2 receptors. When GL cells were challenged with ligands that can bind to both receptor subtypes (PGE(2) and 16,16 dimethyl PGE(2)) or exclusively to EP2 (butaprost), both cAMP formation and progesterone synthesis were stimulated. Furthermore, the cAMP response to these agonists could be significantly blocked by an EP1/2 antagonist AH6809 but not by an EP1-selective antagonist SC19220. Exposure of GL cells to 16,16-dm PGE(2) transiently raised [Ca(2+)](i) levels, which could be prevented by both AH6809 and SC19220. We therefore conclude that human GL cells express functional EP1 and EP2 receptors.
- Oxford University Hospitals NHS Trust United Kingdom
- University College London United Kingdom
- John Radcliffe Hospital United Kingdom
- University of Warwick United Kingdom
- Royal Veterinary College United Kingdom
16-Dimethylprostaglandin E2, luteal cell, Intracellular Fluid, receptor binding, Messenger, 8-chloro-, f1, f)(1, 16,16-Dimethylprostaglandin E2, Receptors, Cyclic AMP, 2-acetylhydrazide, Dibenz(b, Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazide, Cells, Cultured, Progesterone, Cultured, Prostaglandin E, article, granulosa cell, Receptors, Prostaglandin E, EP1 Subtype, unclassified drug, priority journal, Female, Drug, signal transduction, 570, Prostaglandin Antagonists, Cells, Xanthones, prostaglandin ep 1 receptor, progesterone, receptor subtype, Dinoprostone, Dose-Response Relationship, reverse transcription polymerase chain reaction, 11 dihydrodibenzb, Humans, Receptors, Prostaglandin E, cyclic AMP, human, RNA, Messenger, Alprostadil, protein expression, prostaglandin E2, Granulosa Cells, Dose-Response Relationship, Drug, 1 acetyl 2 (8 chloro 10, human cell, prostaglandin ep 2 receptor, Lutein, 16, Receptors, Prostaglandin E, EP2 Subtype, 4)oxazepine-10(11H)-carboxylic acid, 6 isopropoxy 9 oxo 2 xanthenecarboxylic acid, Xanthenes, prostaglandin receptor, C990 - Biological sciences not elsewhere classified, RNA, Calcium, 4oxazepine 10 carbonyl)hydrazine
16-Dimethylprostaglandin E2, luteal cell, Intracellular Fluid, receptor binding, Messenger, 8-chloro-, f1, f)(1, 16,16-Dimethylprostaglandin E2, Receptors, Cyclic AMP, 2-acetylhydrazide, Dibenz(b, Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazide, Cells, Cultured, Progesterone, Cultured, Prostaglandin E, article, granulosa cell, Receptors, Prostaglandin E, EP1 Subtype, unclassified drug, priority journal, Female, Drug, signal transduction, 570, Prostaglandin Antagonists, Cells, Xanthones, prostaglandin ep 1 receptor, progesterone, receptor subtype, Dinoprostone, Dose-Response Relationship, reverse transcription polymerase chain reaction, 11 dihydrodibenzb, Humans, Receptors, Prostaglandin E, cyclic AMP, human, RNA, Messenger, Alprostadil, protein expression, prostaglandin E2, Granulosa Cells, Dose-Response Relationship, Drug, 1 acetyl 2 (8 chloro 10, human cell, prostaglandin ep 2 receptor, Lutein, 16, Receptors, Prostaglandin E, EP2 Subtype, 4)oxazepine-10(11H)-carboxylic acid, 6 isopropoxy 9 oxo 2 xanthenecarboxylic acid, Xanthenes, prostaglandin receptor, C990 - Biological sciences not elsewhere classified, RNA, Calcium, 4oxazepine 10 carbonyl)hydrazine
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