Identification of two distinct functions for TGF-β in early mouse development
Copyright policy )Identification of two distinct functions for TGF-β in early mouse development
In this study the function of transforming growth factor-beta (TGF-beta) in preimplantation mouse embryos was examined. By RT-PCR, mRNA for the signalling type I (T beta R-I) and type II (T beta R-II) receptors for TGF-beta was shown to be present in two distinct time windows: in fertilized oocytes and at the blastocyst stage. The function of TGF-beta at these times was analysed in two ways. Firstly, the TGF-beta signalling pathway was blocked by injecting a DNA construct encoding a truncated T beta R-II, that acts as a dominant-negative receptor, in fertilized oocytes, and the effect on development was determined. Secondly, inner cell masses isolated at the blastocyst stage were cultured in vitro with and without TGF-beta under conditions that favour the outgrowth of parietal endoderm. The results show that TGF-beta signalling mediated by maternally expressed receptors is important for development of preimplantation embryos beyond the two-cell stage, and suggest a regulatory role for TGF-beta in the outgrowth of parietal endoderm.
Male, Microinjections, Endoderm, Receptor, Transforming Growth Factor-beta Type I, Receptor, Transforming Growth Factor-beta Type II, Protein Serine-Threonine Kinases, Fibronectins, Mice, Inbred C57BL, Embryonic and Fetal Development, Mice, Blastocyst, Organ Culture Techniques, Mice, Inbred CBA, Animals, Female, RNA, Messenger, Activin Receptors, Type I, Receptors, Transforming Growth Factor beta, Sequence Deletion, Signal Transduction
Male, Microinjections, Endoderm, Receptor, Transforming Growth Factor-beta Type I, Receptor, Transforming Growth Factor-beta Type II, Protein Serine-Threonine Kinases, Fibronectins, Mice, Inbred C57BL, Embryonic and Fetal Development, Mice, Blastocyst, Organ Culture Techniques, Mice, Inbred CBA, Animals, Female, RNA, Messenger, Activin Receptors, Type I, Receptors, Transforming Growth Factor beta, Sequence Deletion, Signal Transduction
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