descriptionPublicationkeyboard_double_arrow_right Article 15 Jan 1999 English Publisher:WileyJournal:Yeast, volume 15, pages 63-72 (issn: 0749-503X, eissn: 1097-0061,

Authors: Dueñas, Encarnación; Revuelta Doval, José Luis; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R.;

doi: 10.1002/(sici)1097-0061(19990115)15:1<63::aid-yea338>3.0.co;2-7 , 10.1002/(sici)1097-0061(19990115)15:1<63::aid-yea338>3.3.co;2-z

pmid: 10028186

handle: 10261/66357

Disruption and basic phenotypic analysis of six novel genes from the left arm of chromosome XIV ofSaccharomyces cerevisiae

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Abstract

We describe here the construction of six deletion mutants and their basic phenotypic analysis in three different backgrounds. The six genes were disrupted in three diploid strains (FY1679, W303 and CEN.PK2) by the long flanking homology (LFH) method (Wach, 1996). Transformants were selected as geneticin (G418)-resistant colonies and correct integration of the kanMX4 cassette was checked by colony PCR. Following sporulation of the heterozygous diploids, tetrads were dissected and scored for segregation of G418-resistance and auxotrophic markers. One of the six ORFs (YNL158w) corresponds to an essential gene which has no homology with other genes present in the databases and has two predicted transmembrane domains. Growth tests performed on different media at 15 degrees C, 30 degrees C or 37 degrees C with haploid deletants of the five non-essential genes revealed no apparent phenotype in any of them.

Related Organizations

Spanish National Research Council
Spain
University of Salamanca
Spain

Keywords

Phenotype, Transformation, Genetic, Genes, Fungal, Restriction Mapping, Saccharomyces cerevisiae, Chromosomes, Fungal, Polymerase Chain Reaction, Gene Deletion, Plasmids

14 Research products, page 1 of 2

Functional analysis of six ORFs from Saccharomyces cerevisiae chromosome IV: two‐spored asci produced by disruptant of YDR027c and strain‐dependent DNA heterogeneity around YDR036c
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