We have studied the roles of PhyA, PhyB and CRY1 photoreceptors and the downstream light-signaling components, COP1 and DET1, in mediating high-irradiance light-controlled activity of promoters containing synthetic light-responsive elements (LRE). Promoters with paired LREs were able to respond to a wide spectrum of light through multiple photoreceptors, while the light-inducible single LRE promoters primarily responded to a specific wavelength of light. In addition, our results indicate that Cry1 is involved in PhyB-mediated red-light induction of the G-GATA/NOS101 promoter, and that both Cry1 and PhyB are required for effective repression of the GT1/NOS101 promoter by red or blue light. An interaction between PhyA and PhyB in mediating GT1-GATA/NOS101 promoter light activation was also observed. Furthermore, our data indicate that COP1 and DET1 exert negative control in the dark only on paired LRE promoters but not single LRE promoters. From these results, we conclude that the combinatorial interaction of LREs is essential in determining the ability of light-responsive promoters to be modulated by crucial cellular regulators and to respond to diverse light environments.