Stem Cell Factor/c-kit Receptor Signaling Enhances the Proliferation and Invasion of Colorectal Cancer Cells Through the PI3K/Akt Pathway
Copyright policy )Stem Cell Factor/c-kit Receptor Signaling Enhances the Proliferation and Invasion of Colorectal Cancer Cells Through the PI3K/Akt Pathway
In this study, we examined the role of c-kit receptor (KIT) signal transduction on the proliferation and invasion of colorectal cancer cells. We found that c-kit was expressed in 2 colorectal cancer cell lines as determined by RT-PCR, Western blot, and flow cytometry. In KIT-positive lines, KIT was activated by stem cell factor (SCF). SCF enhanced cellular proliferation of positive lines as demonstrated by the WST-1 proliferation assay. Furthermore, SCF enhanced the invasive ability of KIT-positive cell lines. SCF stimulation upregulated p44/42 mitogen-activated protein kinase (MAPK) and Akt as shown by Western blot. We examined the roles played by p44/42 MAPK and phosphatidylinositol 3-kinase (PI3K)/Akt pathways in proliferation and invasion. PI3K/Akt activity strongly correlated with proliferation and invasion and p44/42 MAPK was correlated with only invasion. In conclusion, the SCF-enhanced proliferation and invasion of KIT-positive colorectal cancer cells is achieved mainly through the PI3K/Akt pathway.
- Nagoya City University Japan
Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Protein-Tyrosine Kinases, Flow Cytometry, Piperazines, Gene Expression Regulation, Neoplastic, Phosphatidylinositol 3-Kinases, Pyrimidines, Cell Line, Tumor, Benzamides, Imatinib Mesylate, Humans, Neoplasm Invasiveness, Phosphorylation, Colorectal Neoplasms, Protein Kinase Inhibitors, Cell Proliferation, Signal Transduction
Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Protein-Tyrosine Kinases, Flow Cytometry, Piperazines, Gene Expression Regulation, Neoplastic, Phosphatidylinositol 3-Kinases, Pyrimidines, Cell Line, Tumor, Benzamides, Imatinib Mesylate, Humans, Neoplasm Invasiveness, Phosphorylation, Colorectal Neoplasms, Protein Kinase Inhibitors, Cell Proliferation, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).69 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!