Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V
Copyright policy )Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V
Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.
- Boston Biomedical Research Institute United States
- University of Liège Belgium
Models, Molecular, Myosin Light Chains, Saccharomyces cerevisiae Proteins, Protein Conformation, Molecular Sequence Data, Myosin Type V, Saccharomyces cerevisiae, X-Ray Diffraction/methods, Biochimie, biophysique & biologie moléculaire, Support, U.S. Gov't, P.H.S., Support, U.S. Gov't, Non-P.H.S., X-Ray Diffraction, Escherichia coli, Recombinant Proteins/chemistry/isolation & purification/metabolism, Amino Acid Sequence, Cloning, Molecular, Saccharomyces cerevisiae Proteins/chemistry/isolation &, purification/metabolism, Binding Sites, Myosin Type V/*chemistry/metabolism, Life sciences, Recombinant Proteins, Sciences du vivant, Saccharomyces cerevisiae/growth & development/physiology, Cell Division, Myosin Light Chains/*chemistry/isolation & purification/metabolism, Biochemistry, biophysics & molecular biology
Models, Molecular, Myosin Light Chains, Saccharomyces cerevisiae Proteins, Protein Conformation, Molecular Sequence Data, Myosin Type V, Saccharomyces cerevisiae, X-Ray Diffraction/methods, Biochimie, biophysique & biologie moléculaire, Support, U.S. Gov't, P.H.S., Support, U.S. Gov't, Non-P.H.S., X-Ray Diffraction, Escherichia coli, Recombinant Proteins/chemistry/isolation & purification/metabolism, Amino Acid Sequence, Cloning, Molecular, Saccharomyces cerevisiae Proteins/chemistry/isolation &, purification/metabolism, Binding Sites, Myosin Type V/*chemistry/metabolism, Life sciences, Recombinant Proteins, Sciences du vivant, Saccharomyces cerevisiae/growth & development/physiology, Cell Division, Myosin Light Chains/*chemistry/isolation & purification/metabolism, Biochemistry, biophysics & molecular biology
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