A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
Copyright policy )A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
AbstractOff-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
- Harvard University United States
- Howard Hughes Medical Institute United States
- Harvard Medical School United States
- Boston Biomedical Research Institute United States
- New York University United States
570, Genome, Models, Genetic, Transcription, Genetic, Method, Genes, Insect, Sensitivity and Specificity, Gene Expression Regulation, Genetic Techniques, Animals, Drosophila Proteins, RNA, Drosophila, RNA Interference, Gene Library, RNA, Double-Stranded
570, Genome, Models, Genetic, Transcription, Genetic, Method, Genes, Insect, Sensitivity and Specificity, Gene Expression Regulation, Genetic Techniques, Animals, Drosophila Proteins, RNA, Drosophila, RNA Interference, Gene Library, RNA, Double-Stranded
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